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聚乙烯亚胺,线性PEI,Polyethylenimine, Linear, MW 25000, Transfection Grade

PEI 25K is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale .

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市场价 ¥2200.00

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2000

数量54

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cmorn

产品规格

1g

货号

KE1075

数量

(库存54件)

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  • 产品参数
  • 品牌cmorn

  • 产品名称聚乙烯亚胺,线性PEI,Polyethylenimine, Linear, MW 25000, Transfection Grade

  • 产品货号KE1075

  • 产品规格1g

  • 保存温度室温

聚乙烯亚胺,线性PEI,Polyethylenimine, Linear, MW 25000, Transfection Grade

PEI 25K is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). Each year, more researchers and companies turn to Polysciences PEI to gain a critical edge in their work. Relative to most other options, using PEI to prepare transfection reagents in-house can offer as much as a 40% reduction in total transfection costs.

CAS#: 9002-98-6, 26913-06-4

Formula: (CH2CH2NH)n

Molecular Weight: 25,000

Melting Point: 73-75º 

Soluble In: hot water, cold water at low pH, methanol, and ethanol.

Insoluble In: benzene, ethyl ether and acetone

Appearance: White to yellow solid

Reference(s):

Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020

Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A.102(16):5679-84.

Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9

TSCA  

Hazards: Unknown

Handling: Gloves & fume hood

Storage: Store at room temperature



仅供研究使用,不用于诊断过程。

Transfection Reagent Preparation and Storage Recommendations


    These guidelines are our recommendations based off research, testing, and customer feedback. There are alternative methods available, but we suggest following these recommendations for easy adoption and consistently high performance. These  methods are adapted from Cold Spring Harbor Protocols, an excellent secondary resource for transient transfection literature.

Reagent Preparation (1 mg/mL)

Materials:

    1.1g PEI 25K (kingmornCatalog# KE1075).

    2.1L Milli-Q® water, water for injection (WFI), or comparable biological-grade water

    3.12 M Hydrochloric Acid (HCl)

    4.10 M Sodium Hydroxide (NaOH)

    5.Disposable 0.1-0.2um PES vacuum sterile-filter.

    6.Sterile HDPE or polypropylene storage vials for reagent aliquots. 

Equipment

    1.1L glass beaker

    2.1L glass graduated cylinder

    3.Calibrated pH meter

    4.2 x disposable 1mL plastic pipettes

    5.Stir plate

    6.PTFE coated stir bar

    7.Vacuum pump

  Method:

    1.Dispense 1g of PEI 25K into beaker and suspend in 900 mL of water.

    2.Add stir bar and set stirring to produce a small vortex.

    3.Add hydrochloric acid dropwise until pH is < 2.0.

    4.Cover top of beaker and stir for up to 3 hours until powder fully dissolves.

    5.Add sodium hydroxide dropwise until pH is 6.9-7.1.

    6.Transfer solution to graduated cylinder and add water until the total volume is 1L.

    7.Sterile-filter through vacuum membrane.

    8.Aliquot as desired and store at -20℃.

  Reagent Storage

    Frozen aliquots can be stored at -20℃ for up to one year. Aliquots can be thawed and kept at 4℃ for up to two weeks, but should never be re-frozen.

  References

    1. Polyethylenimine (PEI), linear (1 mg/mL). Cold Spring Harbor Protocols 2008, pdb.rec11323– pdb.rec11323 (2008). doi:10.1101/pdb.rec11323


暂无评价

产品详情

聚乙烯亚胺,线性PEI,Polyethylenimine, Linear, MW 25000, Transfection Grade

PEI 25K is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). Each year, more researchers and companies turn to Polysciences PEI to gain a critical edge in their work. Relative to most other options, using PEI to prepare transfection reagents in-house can offer as much as a 40% reduction in total transfection costs.

CAS#: 9002-98-6, 26913-06-4

Formula: (CH2CH2NH)n

Molecular Weight: 25,000

Melting Point: 73-75º 

Soluble In: hot water, cold water at low pH, methanol, and ethanol.

Insoluble In: benzene, ethyl ether and acetone

Appearance: White to yellow solid

Reference(s):

Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020

Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A.102(16):5679-84.

Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9

TSCA  

Hazards: Unknown

Handling: Gloves & fume hood

Storage: Store at room temperature



仅供研究使用,不用于诊断过程。



配制方法

Transfection Reagent Preparation and Storage Recommendations


    These guidelines are our recommendations based off research, testing, and customer feedback. There are alternative methods available, but we suggest following these recommendations for easy adoption and consistently high performance. These  methods are adapted from Cold Spring Harbor Protocols, an excellent secondary resource for transient transfection literature.

Reagent Preparation (1 mg/mL)

Materials:

    1.1g PEI 25K (kingmornCatalog# KE1075).

    2.1L Milli-Q® water, water for injection (WFI), or comparable biological-grade water

    3.12 M Hydrochloric Acid (HCl)

    4.10 M Sodium Hydroxide (NaOH)

    5.Disposable 0.1-0.2um PES vacuum sterile-filter.

    6.Sterile HDPE or polypropylene storage vials for reagent aliquots. 

Equipment

    1.1L glass beaker

    2.1L glass graduated cylinder

    3.Calibrated pH meter

    4.2 x disposable 1mL plastic pipettes

    5.Stir plate

    6.PTFE coated stir bar

    7.Vacuum pump

  Method:

    1.Dispense 1g of PEI 25K into beaker and suspend in 900 mL of water.

    2.Add stir bar and set stirring to produce a small vortex.

    3.Add hydrochloric acid dropwise until pH is < 2.0.

    4.Cover top of beaker and stir for up to 3 hours until powder fully dissolves.

    5.Add sodium hydroxide dropwise until pH is 6.9-7.1.

    6.Transfer solution to graduated cylinder and add water until the total volume is 1L.

    7.Sterile-filter through vacuum membrane.

    8.Aliquot as desired and store at -20℃.

  Reagent Storage

    Frozen aliquots can be stored at -20℃ for up to one year. Aliquots can be thawed and kept at 4℃ for up to two weeks, but should never be re-frozen.

  References

    1. Polyethylenimine (PEI), linear (1 mg/mL). Cold Spring Harbor Protocols 2008, pdb.rec11323– pdb.rec11323 (2008). doi:10.1101/pdb.rec11323




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